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目的 :建立检测可溶型TRAIL的ELISA ,并评价其临床应用价值。方法 :采用本室制备的鼠抗TRAIL的单抗 (mAb)FMU1.1作为包被抗体 ,兔的抗TRAIL高效价多抗为夹心抗体 ,建立检测sTRAIL的ELISA ,并测定了 9例肾综合征出血热 (HFRS)患者和 4 0例银屑病患者血清sTRAIL的水平。结果 :建立了由单抗和多抗组成的检测人sTRAIL的夹心ELISA ,灵敏度为 0 .0 8μg/L。 9例肾综合征出血热 (HFRS)患者中有 3例急性期患者血清sTRAIL水平升高 ,4 0例银屑病患者中 8例升高。结论 :成功地建立了一种可用于检测血清sTRAIL的双夹心ELISA ,为判定某些相关疾病患者的病情、疗效及预后提供有用的工具
Objective: To establish a ELISA for the detection of soluble TRAIL and to evaluate its clinical value. Methods: The anti-TRAIL monoclonal antibody (mAb) FMU1.1 was used as coating antibody. The anti-TRAIL high titer and anti-rabbit antibody was used as the sandwich antibody. ELISA for detecting sTRAIL was established and 9 cases of renal syndrome Hemorrhagic fever (HFRS) patients and 40 patients with psoriasis serum sTRAIL levels. Results: Sandwich ELISA for detecting human sTRAIL consisting of monoclonal antibody and polyclonal antibody was established with a sensitivity of 0.08 μg / L. Serum sTRAIL levels increased in 3 of 9 patients with acute renal syndrome (HFRS) and in 8 of 40 patients with psoriasis. CONCLUSIONS: A double-sandwich ELISA that can be used to detect serum sTRAIL has been successfully established to provide a useful tool for judging the condition, efficacy and prognosis of patients with certain related diseases