[目的]通过对沙门菌fimy基因保守片段的焦磷酸测序,达到鉴定沙门菌的目的。[方法]通过短片段基因序列测定检测沙门菌。[结果]通过焦磷酸测序法对fimy基因上的27个碱基片段进行测定,可准确检测191株沙门菌。[结论]建立的方法具有准确、快速和实时检测等优点,适于对沙门菌的快速、高通量检测。 [Objective] The aim of the identification of Salmonella was achieved by pyrosequencing of conserved fragment of fimy gene of Salmonella. [Method] The detection of Salmonella by short fragment gene sequencing. [Result] The detection of 27 base pairs of fimy gene by pyrosequencing method could detect 191 strains of Salmonella accurately. [Conclusion] The established method has the advantages of accurate, rapid and real-time detection and is suitable for rapid and high-throughput detection of Salmonella.