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目的:对二藤通痹合剂进行有效部位提取,并以抗类风湿药理作用验证其合理性,筛选出最优提取部位。方法:制作大鼠关节炎模型(AA模型),以各组提取液灌胃给药,测量大鼠足肿胀度,测定血清细胞因子白介素-2(IL-2)、白介素-6(IL-6),并取后肢关节做病理组织学检查。结果:二藤提取液Ⅰ、Ⅱ、Ⅲ组均对大鼠足肿胀有消炎消肿作用,其中Ⅱ组与模型组比较有统计学意义(P<0.01);二滕提取液Ⅱ、Ⅲ组有减轻关节滑膜病变、减轻关节软骨病变作用(P<0.01);二藤提取液Ⅰ、Ⅲ组能降低AA大鼠血中IL-2、IL-6水平。结论:优选组分Ⅲ为最佳提取液。
OBJECTIVE: To extract effective fractions from Erteng Tongbi Mixture and verify its rationality against pharmacological effects of rheumatoid and screen out the optimal extraction sites. METHODS: A rat model of arthritis (AA model) was prepared. The rats were administrated intragastrically with each group of extracts. The degree of rat foot swelling was measured. Serum cytokines interleukin-2 (IL-2) and interleukin-6 (IL-6) were measured. ), And take hindlimb joints for histopathological examination. RESULTS: All the extracts from the rattan extract group I, II, and III had anti-inflammatory and swelling effects on the paw swelling of the rat, and there was statistical significance between the group II and the model group (P<0.01). The extracts from the II rattan extract group II and III had The joint synovial membrane lesions were alleviated and articular cartilage lesions were alleviated (P<0.01). Ertan extracts I and III reduced the blood IL-2 and IL-6 levels of AA rats. Conclusion: The preferred component III is the best extract.