应用光敏生物素标记DNA探针斑点杂交技术检测了41例不同类型未缓解白血病病人外周血或骨髓细胞中C-myc,N-ras,C-abl,C-fos和C-sis等5种癌基因mRNA的表达水平。结果是在白血病时其过度表达率分别达78.0%,90.2%,78.0%,80.6%和67.7%。10例健康成人均呈弱表达或不表达。细胞核体外转录试验证明白血病细胞中C-myc基因的转录产物mRNA显著高于正常细胞。 Biotin-labeled DNA probes were used to detect the expression of C-myc, N-ras, C-abl, C-fos and C-sis in peripheral blood or bone marrow cells of 41 patients with different types of unrelated leukemia Gene mRNA expression levels. The result was over-expression rates of 78.0%, 90.2%, 78.0%, 80.6% and 67.7% in leukemia patients, respectively. 10 cases of healthy adults were weakly expressed or not expressed. In vitro nuclear transcript assay demonstrated that the transcription product of C-myc gene in leukemia cells was significantly higher than that in normal cells.