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目的建立胃能方有效组分中柚皮苷,橙皮苷,新橙皮苷,柴胡皂苷b2,木香烃内酯和去氢木香内酯6种成分的含量测定方法。方法采用HPLC法,色谱柱:Agilent Eclipse XDB-C18(4.6 mm×250 mm,5μm);流动相:乙腈-0.1%磷酸水;流速:1 mL/min;检测波长:280、254、225 nm。结果柚皮苷平均回收率为103.45%,RSD为0.92%(n=5);橙皮苷平均回收率为98.48%,RSD为2.43%(n=5);新橙皮苷平均回收率为103.60%,RSD为1.75%(n=5);柴胡皂苷b2平均回收率为102.05%,RSD为1.06%(n=5);木香烃内酯平均回收率为99.36%,RSD为1.26%(n=5);去氢木香内酯平均回收率为99.02%,RSD为2.24%(n=5)。结论 3批样品测定结果表明,该方法简便,准确,可用于胃能方有效组分中柚皮苷等6种成分的含量测定。
OBJECTIVE To establish a method for the determination of six components of naringin, hesperidin, neohesperidin, saikosaponin b2, linalool lactone and dehydrocostuslane in Weoneng effective component. Methods The HPLC method was used. The column was Agilent Eclipse XDB-C18 (4.6 mm × 250 mm, 5 μm). The mobile phase was acetonitrile -0.1% phosphoric acid water. The flow rate was 1 mL / min. The detection wavelength was 280,254,225 nm. Results The average recovery of naringin was 103.45% with RSD of 0.92% (n = 5). The average recovery of hesperidin was 98.48% with RSD of 2.43% (n = 5). The average recovery of neohesperidin was 103.60 , RSD was 1.75% (n = 5). The average recoveries of saikosaponin b2 were 102.05% with RSD 1.06% (n = 5) n = 5). The average recovery of dehydrovaryllactone was 99.02% with a RSD of 2.24% (n = 5). Conclusion The results of three batches of samples show that the method is simple and accurate and can be used to determine the content of six components such as naringin in the effective components of Weinang prescription.