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验［１］：颈椎脱臼处死小鼠，腹腔内注入５ｍｌ冷Ｈａｎｋ液，轻揉腹部数分钟后，解剖吸取腹腔液，装入含肝素的离心管中，调整细胞为５×１０６个／ｍｌ，于９６孔细胞培养板中，每孔加入１００μｌ，３７℃、５％ＣＯ２温箱中孵育４ｈ使其贴壁。用１０％ＦＣＳ－ＲＰＭ...
Test [1]: The mice were sacrificed by cervical dislocation, and 5 ml of cold Hank fluid was injected intraperitoneally. After gently rubbing the abdomen for several minutes, the peritoneal fluid was dissected and loaded into heparin-containing centrifuge tubes to adjust the cells to 5×10 6 cells/ml. In a 96-well cell culture plate, 100 μl per well was added and incubated for 4 h at 37° C. in a 5% CO 2 incubator to adhere to the well. With 10% FCS-RPM...