采用平板稀释法进行菌种选育,筛选玉米茎腐病的生防菌及对该菌株进行液体发酵。应用响应面法优化发酵条件,以禾谷镰刀菌为指示菌测定生物活性。筛选得到1株较高活性菌株(YH-1),将该菌株进行发酵条件优化,抑菌圈直径由原来的20.60 mm增至25.80 mm。YH-1作为高效玉米茎腐病拮抗菌株,可为生防菌剂开发奠定良好基础。 The method of plate dilution was used to select the strains and the biocontrol bacteria of corn stalk rot were selected and the strain was liquid fermented. Response surface methodology was used to optimize the fermentation conditions, and Fusarium graminearum was used as the indicator to determine the bioactivity. One highly active strain (YH-1) was screened and the fermentation conditions were optimized. The diameter of inhibition zone increased from 20.60 mm to 25.80 mm. YH-1, as an antagonistic strain of corn stalk rot, can lay a good foundation for the development of biocontrol agents.