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目的:利用动物模型,观察助阳通络方不同剂量组对乳腺增生病模型大鼠性激素水平的影响以及雌、孕激素受体表达情况,探讨其作用机制。方法:采用放射免疫法测定血清雌二醇(E2)、孕酮(P)、催乳素(PRL)的激素水平;采用免疫组化法测定乳腺增生病模型大鼠雌激素受体(ER)、孕激素受体(PR)阳性表达。结果:在助阳通络方对乳腺增生病大鼠性激素水平的影响实验中,助阳通络方高剂量组(E组)与正常对照组(A组)比较,E2、P、PRL均有显著性差异(P<0.01);与疾病模型组(B组)比较,E2、P、PRL均有显著性差异(P<0.01)。助阳通络方中剂量组(F组)与A组比较,无显著性差异(P>0.05);与B组比较E2、P、PRL均有显著性差异(P<0.01)。助阳通络方低剂量组(G组)与A组比较,E2、P、PRL均有显著性差异(P<0.01);与B组比较E2、P、PRL均有显著性差异(P<0.01)。在助阳通络方对乳腺增生病大鼠ER、PR的影响实验中,E组与A组比较,无显著性差异(P>0.05);与B组比较,无显著性差异(P>0.05)。F组与A组比较,无显著性差异(P>0.05);与B组比较ER、PR阳性表达显著降低(P<0.01)。G组与A组比较,ER阳性表达显著升高(P<0.01);PR阳性表达升高(P<0.05);与B组比较,无显著性差异(P>0.05)。结论:助阳通络方可降低造模大鼠乳腺组织中升高的E2、PRL的含量,升高造模大鼠乳腺组织中降低的P含量,与三苯氧胺作用类似。可使造模大鼠乳腺组织中ER、PR阳性表达受抑制,且作用与三苯氧胺类似。
OBJECTIVE: To observe the effect of different dosage groups of “Yang-Yang Tongluo Prescription” on the levels of sex hormones and the expression of estrogen and progesterone receptors in rats with mammary gland hyperplasia and to explore its mechanism. Methods: The levels of serum estradiol (E2), progesterone (P) and prolactin (PRL) were determined by radioimmunoassay. The levels of estrogen receptor (ER) Progesterone receptor (PR) positive expression. Results: In the experiment of influencing the sex hormones of rats with hyperplasia of mammary glands, the group of high-dose of Yang-Yang Tongluo Prescription (group E) compared with the normal control group (group A), E2, P and PRL (P <0.01). Compared with the disease model group (B group), E2, P and PRL had significant difference (P <0.01). There was no significant difference (P> 0.05) between the middle dose Yangxuetongluo formula group (F group) and A group (P <0.01). There was significant difference of E2, P and PRL between low dose group (group G) and group A (P <0.01), but there was significant difference between group B and group B (P < 0.01). There was no significant difference between group E and group A (P> 0.05), while there was no significant difference between group B and group B (P> 0.05) ). There was no significant difference between group F and group A (P> 0.05). Compared with group B, the expression of ER and PR significantly decreased (P <0.01). Compared with group A, the expression of ER in G group was significantly increased (P <0.01); the expression of PR was increased (P <0.05); there was no significant difference between group G and group B (P> 0.05). Conclusion: YYTT can reduce the content of E2 and PRL in the mammary gland of rats and increase the content of P in breast tissue of the model rats, which is similar to that of tamoxifen. It can inhibit the expression of ER and PR in the mammary gland of rats, and the effect is similar to that of tamoxifen.