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目的：探讨人脑胶质瘤组织中三种耐药基因 mdr1、 ERCC2和 MGMT mRNA表达之间的相关性。方法：采用双管 RT- PCR方法检测 14例正常脑组织和 56例人脑胶质瘤组织中 mdr1、 ERCC2和 MGMT mRNA水平，并分析其相关性。结果：正常脑组织中 mdr1、 ERCC2和 MGMT的表达量分别为 (0.75± 0.36)， (1.43± 0.92)和 (2.48± 1.83)， mdr1和 ERCC2、 ERCC2和 MGMT之间具相关性 (P< 0.01和 P< 0.001)；胶质瘤组织中三种基因表达量分别为 (0.53± 0.42)， (1.75± 1.16)和 (2.88± 1.92)， ERCC2和 MGMT之间具极显著相关性 (P< 0.001)。结论： ERCC2和 MGMT基因的表达之间可能具有内在的联系，共同参与细胞对 DNA损伤的修复，并形成耐药；而 mdr1与上述两者之间不具有这种关系。
Objective: To investigate the correlation between mdr1, ERCC2 and MGMT mRNA expressions in human glioma tissues. Methods: The mRNA levels of mdr1, ERCC2, and MGMT in 14 cases of normal brain tissue and 56 cases of human glioma tissues were detected by dual-tube RT-PCR method and their correlations were analyzed. Results: The expression levels of mdr1, ERCC2 and MGMT in normal brain tissue were (0.75±0.36), (1.43±0.92) and (2.48±1.83), respectively, and there was a correlation between mdr1 and ERCC2, ERCC2 and MGMT (P<0.01). And P<0.001); The expression levels of three genes in glioma tissues were (0.53±0.42), (1.75±1.16) and (2.88±1.22), respectively, and there was a highly significant correlation between ERCC2 and MGMT (P<0.001). ). Conclusions: There may be an intrinsic link between the expression of ERCC2 and MGMT genes, which together participate in the repair of DNA damage and the formation of drug resistance; and mdr1 does not have this relationship with the above two.