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目的:观察他莫昔芬耐药乳腺癌细胞BCAR3的表达与上皮间质转化(EMT)现象和乳腺癌的迁移侵袭的关系。探讨他莫昔芬耐药和乳腺癌细胞EMT现象的相关性。方法:以实时定量PCR和Western blot检测乳腺癌上皮样和间质样细胞系中BCAR3的表达;Western blot检测乳腺癌MCF-7、MCF-BCAR3和BT-549中介导EMT发生的转录因子Twist、上皮性标记基因E-钙黏素(E-cadherin)和间质性标记基因N-钙黏素(N-cadherin)表达的改变情况。Transwell侵袭实验检测乳腺癌MCF-7、MCF-BCAR3和BT-549侵袭转移能力。结果:BCAR3在乳腺癌上皮样细胞系中的表达明显低于间质样细胞系;E-cadherin蛋白在MCF-7细胞中的表达为阳性,Twist和N-cadherin表达为阴性;他莫昔芬耐药的细胞MCF-BCAR3 E-cadherin蛋白表达缺失,而Twist和N-cadherin表达阳性。BCAR3过度表达导致乳腺癌细胞MCF-7迁移和侵袭能力增强。结论:过度表达BCAR3增强乳腺癌细胞迁移和侵袭能力,并对他莫昔芬治疗产生耐药,可能与BCAR3诱导乳腺癌上皮间质转化有关。
Objective: To investigate the relationship between the expression of BCAR3 and the epithelial-mesenchymal transition (EMT) in breast cancer and the invasion and metastasis of breast cancer. To investigate the correlation between tamoxifen resistance and EMT in breast cancer cells. Methods: The expression of BCAR3 in breast cancer epithelial and stromal cell lines was detected by real-time PCR and Western blot. Western blot was used to detect the expression of transcription factor Twist in MCF-7, MCF-BCAR3 and BT-549 , The expression of E-cadherin and N-cadherin in epithelial marker gene. Transwell invasion assay was used to detect the invasion and metastasis of breast cancer MCF-7, MCF-BCAR3 and BT-549. Results: The expression of BCAR3 in breast cancer epithelial cell line was significantly lower than that in mesenchyme-like cell line. The expression of E-cadherin protein in MCF-7 cells was positive, while the expression of Twist and N-cadherin was negative. The resistant cells MCF-BCAR3 E-cadherin protein expression is missing, while Twist and N-cadherin expression was positive. BCAR3 overexpression leads to increased migration and invasion of breast cancer cells MCF-7. CONCLUSION: BCAR3 overexpression enhances the ability of breast cancer cells to migrate and invade and is resistant to tamoxifen treatment, which may be related to BCAR3-induced epithelial-mesenchymal transition in breast cancer.