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目的:进一步表征芪芍方有效部位中异黄酮类化合物的含量,建立HPLC法测定芪芍方有效部位中芒柄花素含量的方法。方法:采用ODSHYPERSIL色谱柱(4.6 mm×250 mm,5μm),乙腈-2‰磷酸水梯度洗脱,柱温35℃,流速1 mL.min-1,检测波长249 nm。结果:芒柄花素的线性回归方程为Y=6×106X-16 320(r=0.999 8),表明芒柄花素在0.036 48～0.364 8μg,线性关系良好。平均加样回收率为98.70%,RSD 0.77%。3批芪芍方有效部位中芒柄花素的平均含量分别为0.81,0.74,0.63 mg.g-1。结论:方法简便、准确、重复性好,可以用于芪芍方有效部位中芒柄花素的质量控制。为芪芍方有效部位中异黄酮类化合物质量控制方法的建立提供支撑,同时为有效控制芪芍方有效部位质量奠定基础。
OBJECTIVE: To further characterize the content of isoflavones in the effective fractions of Qi Shao Fang, and to establish a HPLC method for the determination of the content of tomentin in the effective fractions of Qi Shao Fang. Methods: ODSHYPERSIL column (4.6 mm × 250 mm, 5 μm) was used to elute acetonitrile-2 ‰ phosphoric acid. The column temperature was 35 ℃ and the flow rate was 1 mL · min-1. The detection wavelength was 249 nm. Results: The linear regression equation of the formononetin was Y = 6 × 106X-16 320 (r = 0.999 8), showing that the formononetin had a good linear relationship at 0.036 48-0.484 8 μg. The average recovery rate was 98.70%, RSD 0.77%. The average content of the formononetin in the effective parts of the three batches of Qi Qifan Fang was 0.81, 0.74 and 0.63 mg.g-1, respectively. Conclusion: The method is simple, accurate and reproducible. It can be used for the quality control of the formonaton in the effective part of Qishao Fang. Which provided support for establishing the quality control method of isoflavones in the effective fractions of Qi Shao Fang, and laid a foundation for effectively controlling the quality of the effective fractions of Qi Shao Fang.