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目的探讨外源性核苷酸对孕期饮酒C57BL/6J小鼠子代生长发育的影响。方法 C57BL/6J孕鼠随机分为空白对照组、酒精对照组和低(0.01%)、中(0.04%)、高(0.16%)3个核苷酸干预组,对照组动物喂饲普通小鼠饲料,核苷酸干预组喂饲含有相应剂量核苷酸的饲料,并于孕6~15d给予酒精对照组和各干预组孕鼠5g/kgbw酒精灌胃,观察母鼠和仔鼠整个实验周期内体重、进食量、食物利用率的变化和子代小鼠生长发育、神经发育指标达标的时间。结果 0.04%核苷酸干预组孕鼠孕期的体重增长值明显高于酒精对照组(P﹤0.05),3个核苷酸干预组孕鼠的食物利用率也显著高于酒精对照组(P﹤0.01);断乳后4周内,酒精对照组子代小鼠的体重增长值和食物利用率均明显低于其他各组(P﹤0.05和P﹤0.01);酒精对照组子代小鼠出生后门齿萌出、睁眼的时间和平面翻正、悬崖回避、空中翻正等神经反射达标的时间也明显比其他各组迟缓(P﹤0.05和P﹤0.01)。结论孕期补充核苷酸可以干预宫内酒精暴露引起的生长发育迟缓。
Objective To investigate the effect of exogenous nucleotides on the growth and development of offspring of C57BL / 6J mice during pregnancy. Methods C57BL / 6J pregnant rats were randomly divided into blank control group, alcohol control group and low (0.01%), medium (0.04%) and high (0.16%) three nucleotide intervention groups. The feed and nucleotide intervention groups were fed with the feed containing the corresponding dose of nucleotide, and the alcohol control group was given 6 to 15 days of gestation and the alcohol of 5 g / kg bw in each intervention group was given to the rats for the whole experiment period Body weight, food intake, changes in food utilization and offspring mice growth and development, neurodevelopmental indicators of compliance time. Results The body weight gain of pregnancy pregnant rats in 0.04% nucleotide intervention group was significantly higher than that of alcohol control group (P <0.05), and the food utilization rate of pregnant rats in 3 nucleotide intervention group was also significantly higher than that of alcohol control group (P < 0.01). After 4 weeks of weaning, the body weight gain and food utilization rate of offspring of alcohol control group were significantly lower than those of other groups (P <0.05 and P <0.01) The time of echinococcocutaneous eruption, the time of open eyes, the level of face inversion, the avoidance of cliffs and the correction of air reflexes were also significantly slower than those of other groups (P <0.05 and P <0.01). Conclusion Supplementation of nucleotides during pregnancy may prevent growth retardation caused by intrauterine alcohol exposure.