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目的研究中药有效成分人参皂甙Rg1、肉桂酸CINN及丹参酮TanⅡA的组合(简称RCT),诱导人成骨肉瘤MG-63细胞分化过程中,细胞核基质构型与蛋白质组成的变化。方法应用流式细胞仪检测细胞周期变化,用四甲基偶氮唑蓝(MTT)法绘制细胞生长曲线,以选择性抽提整装光镜与电镜技术及蛋白质组学技术分析观察处理前后MG-63细胞核基质构型与蛋白质组成的变化。结果生长曲线显示,RCT处理后MG-63细胞的增殖受到明显抑制,增殖抑制率在第7天达到72.37%,细胞周期出现明显的G0/G1期阻滞。MG-63细胞核基质为典型的肿瘤细胞恶性表型,在RCT处理后发生了向正常细胞转变的恢复性变化。RCT处理后的细胞中多种核基质功能蛋白的表达发生了变化。结论RCT能显著抑制体外培养MG-63细胞的增殖活动,将细胞周期阻滞于G0/G1期,诱导核基质构型发生了显著的恢复性变化,并改变核基质蛋白的组成,对MG-63细胞具有显著的分化诱导作用。
Objective To study the changes of nuclear matrix configuration and protein composition during the differentiation of human osteosarcoma MG-63 cells induced by the combination of ginsenoside Rg1, cinnamic acid CINN and tanshinone TanⅡA (RCT). Methods The changes of cell cycle were detected by flow cytometry. The cell growth curve was drawn by MTT method. The growth curve of MG was observed by selective extraction of whole-mount light and electron microscopy and proteomics techniques -63 nuclear matrix configuration and protein composition change. Results The growth curve showed that the proliferation of MG-63 cells was significantly inhibited after RCT treatment, the proliferation inhibition rate reached 72.37% on the 7th day, and the cell cycle showed obvious G0 / G1 phase arrest. The MG-63 nuclear matrix is a typical malignant phenotype of tumor cells and undergoes a recurrent change to normal cell transformation following RCT treatment. The expression of multiple nuclear matrix functional proteins in RCT-treated cells changed. Conclusion RCT can significantly inhibit the proliferation of MG-63 cells in vitro, block the cell cycle in G0 / G1 phase, and induce a significant recoverable change of nuclear matrix configuration, and change the composition of nuclear matrix protein, 63 cells have a significant induction of differentiation.