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用逆相蒸发法制备黄芩素脂质体,4000r/min,离心10min,分离含药脂质体和游离的药物,稀释后测定其上清和沉淀吸光度,计算其浓度。由包封率=(总浓度一沉淀浓度)/总浓度X 100%计算包封率。发现溶液的稳定性和精密度都良好,测得脂质体中药物的包封率分别为38.49%,36.64%,39.55%,结果较为接近。说明低速离心法是一种有效的水不溶性药物脂质体包封率的测定方法。
The baicalein liposomes were prepared by reverse phase evaporation, centrifuged at 4000r / min for 10min, drug-containing liposomes and free drug were separated, the supernatant and the absorbance of the precipitate were determined after dilution, and the concentration of baicalein was calculated. The encapsulation efficiency was calculated from entrapment ratio = (total concentration-sedimentation concentration) / total concentration X 100%. The stability and precision of the solution were found to be good. The encapsulation efficiency of the drug in the liposomes was 38.49%, 36.64% and 39.55% respectively, and the results were similar. Low-speed centrifugation method is an effective method for determination of liposome encapsulation efficiency of water-insoluble drugs.