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为建立抗青蒿琥酯恶性疟原虫虫株 ,将采自恶性疟病人血 ,用 Trager法进行体外连续培养 ,待其正常生长后 ,在培养基中加不同浓度的青蒿琥酯进行培育 ,并在培育前及用药后不同时间用 Rieckmann体外微量法测定青蒿琥酯半数抑制量 (ID5 0 )。用药前及用药后 6 8d、12 9d及停药后 6 7d的 ID5 0 分别为 9.6、30 .6、85 .1及 5 2 .9nm ol/L。结果表明可用人工方法建立高度抗青蒿琥酯恶性疟原虫虫株 ,停药后抗性程度有所下降
In order to establish anti-artesunate falciparum parahaemolyticus strains, the blood samples were collected from the blood of falciparum malaria patients and were continuously cultured in vitro by the Trager method. After normal growth, artesunate was incubated in different concentrations in the culture medium, The half-inhibition of artesunate (ID50) was determined by Rieckmann in vitro microdialysis before and after incubation. Before treatment and after treatment 6 8d, 12 9d and 6d after stopping ID 5 0 were 9.6,30 .6,85 .1 and 52.9 nmol / L. The results showed that artificial anti-Artesunate Plasmodium falciparum strain could be established by artificial method and the resistance decreased after stopping