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目的:研究逆癌酮诱导A549人肺腺癌细胞系和PLA-801D人肺巨细胞癌细胞系凋亡及其抗癌作用机理。方法:应用体外细胞培养技术,以逆癌酮作用于肺癌细胞,绘制生长曲线,作克隆形成实验,观察其对细胞增殖的影响,并进行光镜和流式细胞术的观察和分析。结果:应用逆癌酮后细胞生长明显受抑,克隆形成能力降低,流式细胞分析表明,应用逆癌酮后,肺癌细胞凋亡指数显著增加(P<0.01),细胞被阻滞于G0/G1期(P<0.01),其bax、Fas的表达被上调;bcl-2的表达被下调(P<0.01);c-myc、TGFβR1的表达也被上调(P<0.01)。结论:逆癌酮对A549细胞和PLA-801D细胞有明显的细胞毒熏并通过上调bax、Fas的表达,下调bcl-2的表达诱导细胞凋亡。
AIM: To study the mechanism of anti-cancer apoptosis induced by anti-cancer inducible A549 human lung adenocarcinoma cell line and PLA-801D human lung giant cell carcinoma cell line. Methods: In vitro cell culture technique was used to effect the growth of lung cancer cells with anti-cancer ketones. The growth curve was drawn and the colony formation assay was performed. The effects on cell proliferation were observed and observed by light microscopy and flow cytometry. Results: The cell growth was obviously inhibited and the colony formation ability was decreased after the application of anti - cancer cell growth factor. Flow cytometry analysis showed that the apoptosis index of lung cancer cells was significantly increased (P <0.01) G1 phase (P <0.01), the expressions of bax and Fas were up-regulated, the expression of bcl-2 was down-regulated (P <0.01), and the expressions of c-myc and TGFβR1 were also up-regulated. CONCLUSION: Inverse anti-cancer cells have obvious cytotoxicity on A549 cells and PLA-801D cells and induce apoptosis by up-regulating the expression of bax and Fas and down-regulating the expression of bcl-2.