论文部分内容阅读
以转透明颤菌血红蛋白基因(vgb)京2杨为材料,采用qRT-PCR分析方法确认其外源基因在转基因无性系中可以正常表达。盆栽试验结果显示,在正常生长条件下转基因无性系的株高和地径显著提高,同时叶片全氮质量分数上升。进而利用非损伤微测技术(Non-invasive Micro-test Technique,NMT)对植株根部NO3-吸收情况的检测发现,在含有0.5mmol/L NO3-测试液中转基因无性系NO3-吸收速率大于非转基因植株。此外,研究还发现,转基因无性系的硝酸还原酶(nitrate reductase,NR)活性显著提高。可见:vgb的表达能提高杨树的氮素利用能力。
The transgenic gerbil hemoglobin gene (vgb) Jing 2 poplar was used as material, and its foreign gene was confirmed by qRT-PCR analysis in transgenic clones. The results of pot experiment showed that under normal growth conditions, the plant height and diameter of transgenic clones significantly increased, while the total nitrogen content of leaves increased. Furthermore, using the non-invasive Micro-test Technique (NMT) to detect NO3-uptake in the plant roots, the NO3-uptake rate of the transgenic clones in 0.5 mmol / L NO3-test solution was higher than that of non-transgenic Micro- Plant. In addition, the study also found that the transgenic clones nitrate reductase (nitrate reductase, NR) activity was significantly increased. Visible: The expression of vgb can improve poplar nitrogen utilization ability.