目的　用双向电泳技术分析人CD4+ CD2 5 + T细胞和CD4+ CD2 5 -T细胞表达的蛋白质组 ,建立二者之间的差异表达谱。方法　从人脾脏分选CD4+ CD2 5 + T细胞和CD4+ CD2 5 -T细胞 ,用双向电泳技术分析两种细胞蛋白质组的表达差异。结果　分选细胞纯度分别为CD4+ CD2 5 + T细胞 82 .3 % ,CD4+ CD2 5 -T细胞 96.5 % ,双向电泳分析发现有 4个蛋白点明显仅于CD4+ CD2 5 + T细胞检测到表达 ,有 9个蛋白点明显仅于CD4+ CD2 5 -T细胞检测到表达。结论　CD4+ CD2 5 + T细胞和CD4+ CD2 5 -T细胞表达蛋白质组有明显差异 Objective To analyze the proteome expression of human CD4 + CD25 + T cells and CD4 + CD25- T cells by two-dimensional electrophoresis, and to establish a differential expression profile between the two. Methods CD4 + CD25 + T cells and CD4 + CD25-T cells were isolated from human spleen and analyzed by two-dimensional electrophoresis. Results The purity of sorted cells was 82.3% of CD4 + CD25 + T cells and 96.5% of CD4 + CD25-T cells, respectively. There were 4 protein spots detected by two-dimensional electrophoresis in CD4 + CD25 + T cells, Nine protein spots were clearly detected only on CD4 + CD25-T cells. Conclusion The protein expression of CD4 + CD25 + T cells and CD4 + CD25-T cells were significantly different