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观察重复+10Gz暴露对大鼠脑脂质过氧化反应和肝肾功能的影响以及天然抗氧化剂茶多酚(TP)的防护作用。方法24只雄性Wistar大鼠随机等分为三组(n=8):A组(对照组),B组(+10Gz组)和C组(TP组)。B、C组重复+10Gz暴露(每次30s,G增长率约为0.5G/s,3次/d,间隔+1Gz1min,3d/wk,共4wk),而A组仅受+1Gz作用。C组于+Gz实验前约1h灌胃给予TP(200mg/kg),另外两组给予等量蒸馏水。测定脑脂质过氧化反应、肝肾功能和血脂水平。结果与对照组相比,B组大脑皮层匀浆、线粒体和胞浆脂质过氧化反应明显增强(P<0.05),血浆肌酐水平明显升高(P<0.01);而TP对此过氧化反应具有显著抑制作用并明显降低血浆肌酐水平。各组间血清谷丙转氨酶活性、甘油三酯和总胆固醇水平无统计学差异。结论重复高+Gz作用可引起脑自由基损伤并损害肾功能,而天然抗氧化剂茶多酚具有明显的保护作用。
To observe the effects of repeated +10 Gz exposure on lipid peroxidation and liver and kidney function in rats brain and the protective effect of natural antioxidant tea polyphenols (TP). Methods Twenty-four male Wistar rats were randomly divided into three groups (n = 8): A group (control group), B group (+10 Gz group) and C group (TP group). Groups B and C were exposed to + 10Gz repetitions (30s each time, G increased approximately 0.5G / s, 3 times / d, intervals + 1Gz1min, 3d / wk, total 4wk), while group A was affected by + 1Gz only. Group C was fed with TP (200 mg / kg) orally at about 1 h before the + Gz test, and the other two groups were given the same amount of distilled water. Determination of lipid peroxidation, liver and kidney function and blood lipid levels. Results Compared with the control group, the lipid peroxidation of mitochondria and mitochondria in group B was significantly increased (P <0.05) and the level of plasma creatinine was significantly increased in group B (P <0.01) This peroxidation has a significant inhibitory effect and significantly reduces plasma creatinine levels. Serum alanine aminotransferase activity, triglyceride and total cholesterol levels between the two groups showed no significant difference. Conclusion Repeated high + Gz can cause damage to brain free radicals and impair renal function, while natural antioxidant tea polyphenols has obvious protective effect.