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利用 12对AFLP引物 ,对来自于世界 6个国家的 14 6份小豆 (Vignaangularis)栽培变种 (var.angularis)和野生变种 (var.nipponensis)种质的基因组DNA进行扩增 ,得到 5 80条清晰的显带 ,其中 313(5 3 93% )条呈多态性 ,平均每对AFLP引物得到 2 6 0 8条多态性带 ;平均遗传距离 0 35 ,变异幅度为 0 0 0～ 0 87。利用AFLP多态性数据进行的Jaccard’s遗传距离聚类分析绘制的聚类图 ,可将其中的 14 3份种质相互区分开 ,并将其中的 14 5份小豆资源划分成 8个明显不同组群 ,显示小豆种内存在足够的遗传多样性用于资源材料的准确鉴别与分类。 8个组群的遗传多样性表现出十分明显的地域相关性 ,以及遗传类型趋同性。通过各组群内和组群间的遗传距离比较 ,发现世界小豆主要栽培资源以及日本野生、半野生资源中蓄积的遗传多样性较匮乏 ,而储藏于中国野生小豆资源、喜马拉雅地区栽培和野生资源中的遗传多样性较丰富。
Genomic DNA from 14.6 accessions of Vignaangularis cultivars (var. Angularis) and wild-type (var. Nipponensis) from 6 different countries in the world was amplified using 12 pairs of AFLP primers, and 580 clear Of which 313 (5393%) were polymorphic, with an average of 2,680 polymorphic bands per pair of AFLP primers; the average genetic distance was 0 35 and the variation range was 0 0 0 ~ 0 87. Fourteen germplasms were distinguished from each other by using Jaccard’s genetic distance clustering analysis based on AFLP polymorphism data, and 14 5 of them were divided into 8 distinct groups , Showing that there is enough genetic diversity within adzuki bean species for the accurate identification and classification of resource materials. The genetic diversity of the eight cohorts showed very clear regional correlation and genetic type homogeneity. By comparing the genetic distances among the groups and among the groups, it was found that the genetic diversity of the main cultivated resources of Adzuki bean and the wild and semi-wild resources of Japan were relatively scarce. However, the genetic diversity of wild adzuki beans stored in China, the cultivated and wild resources in the Himalayas The genetic diversity in richer.