Beckman 121 MB型氨基酸分析仪与日本、瑞典等国的同类仪器相比,其最大缺点是无滤氨柱,因此对试剂、环境的要求很高,以防氨对分析的污染,但在实际工作中,蒸馏水、缓冲液、试剂及容器都可能带入氨,导致碱性氨基酸段分离效果差。基线向上漂移,出现氨台阶,分析结果差。对此若采用双柱系统 Beckman 121 MB type amino acid analyzer and Japan, Sweden and other countries compared to similar instruments, the biggest drawback is the filter-free ammonia column, so the reagents, the environment is very high, in order to prevent ammonia pollution of the analysis, but in the actual work In distilled water, buffers, reagents, and containers, ammonia may be introduced, resulting in poor separation of basic amino acids. Baseline drift up, ammonia step appears, poor analytical results. In this case if using double column system