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目的检测结直肠癌(colorectal cancer,CRC)患者组织和外周血中细胞间黏附分子-1(ICAM-1)和血管细胞黏附分子-1(VCAM-1)的表达,探讨ICAM-1和VCAM-1的临床意义。方法应用免疫组化SP法测定CRC原发病灶(68例)、非癌正常组织(68例)、结直肠腺瘤组织51例中的ICAM-1和VCAM-1的表达。应用酶联免疫吸附试验(ELISA)检测CRC患者(68例)和健康者(68例)血清中ICAM-1和VCAM-1的含量。结果 CRC原发病灶组织中,ICAM-1和VCAM-1明显高于非癌正常组织及结直肠腺瘤组织,差异有统计学意义(P<0.05)。CRC患者血清中ICAM-1和VCAM-1含量明显高于健康对照组,差异有统计学意义(P<0.05)。ICAM-1和VCAM-1的表达与性别、年龄、肿瘤部位和病理学分级无关,差异无统计学意义(P>0.05);但与淋巴结转移及Dukes分期相关,差异有统计学意义(P<0.05)。结论 CRC患者组织及外周血中ICAM-1和VCAM-1在CRC的发病过程中具有重要作用,对肿瘤的诊断、恶性程度判断和检测病情变化具有重要意义。
Objective To detect the expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in the tissues and peripheral blood of patients with colorectal cancer (CRC) 1 clinical significance. Methods The expression of ICAM-1 and VCAM-1 in primary CRC lesions (68 cases), non-cancerous normal tissues (68 cases) and colorectal adenoma tissues were detected by immunohistochemical SP method. The serum levels of ICAM-1 and VCAM-1 in CRC patients (68 cases) and healthy controls (68 cases) were detected by enzyme-linked immunosorbent assay (ELISA). Results The levels of ICAM-1 and VCAM-1 in CRC primary lesions were significantly higher than those in non-cancerous normal tissues and colorectal adenomas (P <0.05). The serum levels of ICAM-1 and VCAM-1 in CRC patients were significantly higher than those in healthy controls (P <0.05). The expression of ICAM-1 and VCAM-1 was not related to gender, age, tumor location and pathological grade (P> 0.05), but there was a significant difference between lymph node metastasis and Dukes stage (P < 0.05). Conclusion The expression of ICAM-1 and VCAM-1 in CRC patients’ tissues and peripheral blood plays an important role in the pathogenesis of CRC. It is important for the diagnosis of cancer, the judgment of malignancy and the detection of the changes of the disease.