论文部分内容阅读
目的：观察大黄在创伤后系统炎症反应中对肝脏内细胞因子基因表达的影响，揭示大黄“清热解毒”的药理机制。方法：采用大鼠２５％ 体表面积Ⅲ度烫伤模型，应用随机、前瞻性研究方法进行观察。烫伤后１２ 小时经静脉持续输注内毒素予以“二次打击”，总剂量为２０ ｍ ｇ／ｋｇ。观察大黄对肝脏内肿瘤坏死因子（ＴＮＦ α）、白介素 １β（ＩＬ １β）基因表达的影响，以及对门、体静脉血内毒素的清除作用。结果：正常对照组和单纯烫伤组肝脏内ＴＮＦ α、ＩＬ １β无明显表达，“二次打击”后肝内ＴＮＦ α、ＩＬ １β基因表达明显增加；大黄可明显抑制肝脏内ＴＮＦ α、ＩＬ １β基因表达，与正常对照组、单纯烫伤组和烫伤加内毒素组比较均有显著性差异（Ｐ均＜０．００５）。烫伤可使肠道内毒素吸收入血，门静脉血中内毒素含量明显高于体静脉血（Ｐ＜ ０．０５）。“二次打击”后门、体静脉血中内毒素浓度更高，但两者浓度差消失。大黄治疗后门、体静脉血中内毒素浓度显著下降（Ｐ＜０．０１）。结论：大黄可抑制创伤和后继“二次打击”后肝脏内ＴＮＦ α、ＩＬ １β基因表达，促进体内内毒素清除
OBJECTIVE: To observe the effect of rhubarb on cytokine gene expression in liver after traumatic system inflammation, and to reveal the pharmacological mechanism of rhubarb “clearing heat and detoxifying”. METHODS: A 2% body surface area III degree scald model was used in rats and randomized, prospective studies were performed. Intravenous infusion of endotoxin was continued for 12 hours after scalding to give a “second strike” with a total dose of 20 mg/kg. Observe the effects of rhubarb on the expression of tumor necrosis factor (TNF) and interleukin 1β (IL 1β) genes in the liver, and the clearance of endotoxin from portal and systemic blood. Results: There was no significant expression of TNF α and IL 1β in liver of normal control group and simple scald group. The expression of TNF α and IL 1β gene in hepatic tissue was significantly increased after “two strikes”; Rhubarb can significantly inhibit TNF α and IL 1β gene in liver. The expression was significantly different from the normal control group, the simple scald group and the scald plus endotoxin group (P <0.005). Burns can cause toxins in the intestine to be absorbed into the blood, and the endotoxin content in the portal vein blood is significantly higher than that in the body venous blood (P<0.05). The concentration of endotoxin in the backdoor and body venous blood was higher in the “second strike”, but the difference between the two concentrations disappeared. The concentration of endotoxin in the portal and body venous blood decreased significantly after treatment with rhubarb (P<0.01). Conclusion: Rhubarb can inhibit the expression of TNFα and IL-1β in the liver after trauma and subsequent “second strike” and promote the clearance of endotoxin in vivo.