【摘 要】
:
Objective: To study the apoptosis-resistant difference between EBV-BHRF1 gene positive and negative cell lines against 3 kinds of apoptosis inducing factors. Me
【机 构】
:
Department of Biochemistry, Sun Yat-sen University of Medical Sciences, Guangzhou 510089
论文部分内容阅读
Objective: To study the apoptosis-resistant difference between EBV-BHRF1 gene positive and negative cell lines against 3 kinds of apoptosis inducing factors. Methods:These cell lines were cultured in (1) the absence of fetal calf serum (FCS) for 48 h, (2) 43℃ for 10 min, and (3) 2×10-6 mol/L dexamethasone for 24 h. Polymerase chain reaction (PCR), reverse transcription PCR (RT-PCR), and apoptosis inducing and detecting methods were used. Results: EBVBHRF1 gene fragment was amplified from B95-8, Raji and SUNE-1 cell lines with PCR technique, while K562 and YAC cell lines were negative. The results against 3 kinds of apoptosis inducing factors from electron microscopy and agarose gel electrophoresis indicated that apoptosis appeared in BHRF1 gene-negative cell lines while apoptosis were normal in BHRF1 gene-positive cell lines. Conclusion:EBV-BHRF1 gene is one of the important factors in suppressing its host cell’s apoptosis.
其他文献
Objective: A method for separating mRNAs by means of the polymerase chain reaction (differential display mRNA), and identifying the genes related to radiationin
Objective: To study the effect of antisense EGFR RNA on the growth of human glioma cells in vitro and evaluate the feasibility of targeting EGFR gene for gene t
上篇辽河油田超稠油先导试验通过验收rn截至11月6日,辽河油田曙一区先期转入SAGD生产的馆陶试验区4个井组已累计生产612天,生产原油11.98万吨.10月末,中国石油天然气股份有限
研究了一种基于最大模糊熵高斯聚类的实时图像目标跟踪算法:在目标初始信息(位置、速度)已知的情况下,应用最大模糊熵高斯聚类的方法进行跟踪窗内测量点融合,将融合后的点输入到Kalman滤波器中进行预测目标点下一个状态的位置,在预测位置继续开一个跟踪窗进行检测、融合,直至所有图像都被跟踪完为止。理论及实验结果表明,在序列图像情况下该算法能够在保持跟踪实时性的同时,提供较高的跟踪精度。
请下载后查看,本文暂不支持在线获取查看简介。
Please download to view, this article does not support online access to view profile.
建设社会主义新农村,是党的十六届五中全会提出的一项重大历史任务.为认真贯彻落实中央和江苏省委文件精神和盐城市委、市政府全市社会主义新农村建设动员大会的要求,盐城市
颜真卿书史地位的崛起是中国书法史上发起于宋代的一个重大事件。这一事件的发生,不只是艺术上的推陈出新或吐故纳新,而是蕴含着深刻的历史文化意味。对这一事件的探讨,不仅
静力平衡是中职物理教学中的重点章节,与其他章节类似,应当通过理论与实验的结合形式,开展教学活动。在这种背景下,中职物理静力平衡教学设计应当明确教学内容,注重实验教学,
Objective: To detect which members in the connexin gene family are expressed in nasopharyngeal carcinoma (NPC) cell line HNE1, and the mechanism by which those