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以玉米穗粒腐病的主要高原菌串珠镰刀菌FusariummoniliformeSheld为菌源,采用4种接种方法比较结果,以针刺果穗较深处(籽粒与穗轴间)发病最重,花丝喷雾法发病最轻;接种时期以乳熟期接种为好;接种浓度以10×40倍,每视野60个分生孢子接种效果最好;接种量以每果穗接种2.5mL孢子悬浮液发病重,效果好。
Fusarium moniliformeSheld, a major plateau rot of corn ear rot, was used as a bacterial source. Four inoculation methods were used to compare the results. The results showed that the incidence of acupuncture at earliest depth (between kernels and panicles) was the highest, and that of filament spray was the lightest Inoculation period was milch-ripe stage inoculation was better. Inoculation concentration was 10 × 40 times, 60 conidiospores in each field were the best. Inoculation amount was 2.5ml inoculation per spike of spore suspension, and the effect was good.