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实验观察了微量元素锌对大鼠肝微粒体４种脂质过氧化模型的抗氧化作用。脂质过氧化模型分别是由维生素Ｃ（ＶＣ）、异丙基苯氢过氧化物（ＣＨＰ）、ＮＡＤＰＨ－ＣＣｌ４和ＮＡＤＰＨ－ＡＤＰ－Ｆｅ２＋构成激发剂。前３种模型通过比色法测定丙二醛（ＭＤＡ）含量，最后一种模型采用氧电极法测定氧耗量。结果表明，５０～５００μｍｏｌ·Ｌ－１的锌对大鼠肝微粒体脂质过氧化反应有显著抑制作用，并且在一定终反应浓度内有剂量－反应关系。实验为证实锌在自由基损害中的保护作用提供了实验依据，并初步探讨了锌的抗氧化作用的生化机制
Antioxidant effects of trace element zinc on four lipid peroxidation models of rat liver microsomes were observed experimentally. Lipid peroxidation model is composed of vitamin C (VC), cumene hydroperoxide (CHP), NADPH-CCl4 and NADPH-ADP-Fe2 + constitute an activator. The first three models determined malondialdehyde (MDA) content by colorimetric method. The last model used oxygen electrode method to determine oxygen consumption. The results showed that 50 ~ 500μmol·L-1 of zinc on rat liver microsomal lipid peroxidation was significantly inhibited, and in a certain final reaction concentration-dose-response relationship. In order to confirm the protective effect of zinc on free radical damage, the experiment provided the experimental evidence and the biochemical mechanism of the anti-oxidative effect of zinc