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通过测定大花金鸡菊(Coreopsis grandiflora)乙醇提取物及其各萃取部分对植物病原真菌的抑菌活性,寻找大花金鸡菊中的农用活性物质,为其综合开发利用提供科学依据。采用菌丝生长速率法测定对植物病原真菌的抑菌活性的结果,当浓度为2.0 mg.mL-1时,大花金鸡菊乙醇提取物对小麦赤霉病菌(Gibberella zeae)、苹果斑点落叶病菌(Alternaria alternataf.sp.mali)、辣椒炭疽病菌(Colletotrichum capsici)和黄瓜枯萎病菌(Fusariumoxysporumf.sp.cucumerinum)的抑制作用较好,抑制率分别为48.45%、31.94%、23.22%和22.69%;其中,对小麦赤霉病菌和苹果斑点落叶病菌的EC50分别为1.570和2.872 mg.mL-1。大花金鸡菊乙醇提取物不同溶剂萃取物中,正己烷层和水层的抑菌活性较高。正己烷萃取物对苹果腐烂病菌和小麦赤霉病菌的EC50分别为1.111和0.194 mg.mL-1;水层为1.878和1.390 mg.mL-1。
The antibacterial activities of ethanol extracts of Coreopsis grandiflora and its extracts on phytopathogenic fungi were determined, and the agricultural actives in the flowers of Coreopsis chinense were searched to provide a scientific basis for their comprehensive utilization. The mycelium growth rate method was used to determine the antibacterial activity of phytopathogenic fungi. When the concentration was 2.0 mg.mL-1, the ethanolic extracts of Artemisia fischeriensis had significant inhibitory effect on Gibberella zeae, Alternaria alternataf.sp.mali, Colletotrichum capsici and Fusarium oxysporum f.cucumerinum were 48.45%, 31.94%, 23.22% and 22.69%, respectively. Among them, , And EC50 of 1.570 and 2.872 mg.mL-1 for Fusarium graminearum and apple spot-leaf blight respectively. Among the different solvent extracts of ethanol extract of Artemisia scoparia, n-hexane and water had higher antibacterial activity. The EC50 of n-hexane extract to B. rot and F. graminearum were 1.111 and 0.194 mg.mL-1, respectively, and the aqueous layer was 1.878 and 1.390 mg.mL-1.