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目的:探讨消痹方对兔膝骨性关节炎软骨下骨c-jun m RNA的影响。方法:(1)动物分组与药物干预:采用健康6月龄新西兰大白兔64只,按照随机抽签的方法分成2组,即:试验组(32只)、对照组(32只)。造模术后1周,开始对不同的分组来进行灌胃治疗:对于试验组来说,采用的消痹方水溶液剂量为10 m L/d,而对于对照组来说,则只给予同样剂量的生理盐水来进行灌胃操作,术后的1周、6周、9周、12周要对每一组样品进行取材。(2)检测指标:在对相应的指标进行检测时,需要采用X线摄片以及光镜技术来对兔子的软骨下骨部分进行观测,主要是对其骨头的组织形态进行观察。结果:从观测结果当中可以看出,在利用消痹方进行干预的试验组当中,当术后9周和12周以后,样品组的软骨下骨进行X线片观测的时候,发现这些兔子的软骨下骨处发生了不太明显的退变,而对照组的退变则比较明显;而对于光镜观察的结果来说,试验组的兔子当中,发生骨质增生的样本数和程度也都要比对照组轻很多。结论:采用消痹方对样本进行灌胃处理后,可以达到明显的降低兔子软骨下骨重塑速率的目的。
Objective: To investigate the effect of Xiaobi recipe on c-jun m RNA of subchondral bone in rabbit knee osteoarthritis. Methods: (1) Animal grouping and drug intervention: Sixty-four healthy New Zealand white rabbits aged 6 months were randomly divided into two groups: experimental group (32) and control group (32). One week after modeling, different gavages were started for gavage: for the experimental group, the dose of Xiaobi Fang aqueous solution was 10 m L / d, while for the control group only the same dose Of saline to gavage operation, 1 week, 6 weeks, 9 weeks, 12 weeks after each sample to be drawn. (2) detection index: In the detection of the corresponding indicators, you need to use X-ray and light microscopy techniques to observe the subchondral bone of rabbits, mainly to observe the morphology of the bones. Results: From the observation results, it can be seen that in the experimental group using Xiaobi intervention, when the subchondral bone of the sample group was observed by X-ray after 9 weeks and 12 weeks after operation, it was found that these rabbits Subchondral bone occurred less obvious degeneration, while the degeneration of the control group is more obvious; and for the results of light microscopy, the experimental group of rabbits, the number and extent of bone hyperplasia also It is much lighter than the control group. Conclusion: The gavage treatment of the sample with Xiaobi can significantly reduce the rate of subchondral bone remodeling in rabbits.