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Ngn3(neurogenin 3)基因及其直接靶基因NeuroD1(neurogenic differentiation 1)和Islet-1(insulin gene enhancer binding protein-1)是一类胰岛转录因子(islet transcription factors,ITFs)调控基因。Ngn3基因及其转录调控网络在小鼠和人胚胎胰腺内分泌细胞发育、胰岛素的产生和分泌及重编程干细胞或非β细胞生成胰岛素分泌β细胞中起着重要的调控作用。新近的研究发现,INSM1(insulinoma associated 1)和INSM2(insulinoma associated 2)基因家族成员也是Ngn3基因的直接靶基因,初步证明了INSM1/INSM2基因对胰腺内分泌发育可能起重要的调控作用,进一步补充和完善了Ngn3转录调控网络。因此,深入理解Ngn3基因及其转录调控网络,尤其是INSM1/INSM2基因对胰岛发育和成熟胰岛细胞命运的分子调控机制,将有助于研究胰腺内分泌疾病和内分泌发育疾病,特别是糖尿病的病因、治疗和预防。为此,就近年来Ngn3基因及其转录调控网络的研究进展作一综述。
The neurogenin 3 (Ngn3) gene and its direct target genes, NeuroD1 (neurogenic differentiation 1) and Islet-1 (insulin gene enhancer binding protein-1), are islet transcription factors (ITFs) regulatory genes. The Ngn3 gene and its transcriptional regulatory network play an important regulatory role in the development of endocrine cells in the pancreas of mice and human embryos, insulin production and secretion, and reprogramming of insulin-producing β cells by reprogrammed stem cells or non-β cells. Recent studies have found that INSM1 (insulinoma associated 1) and INSM2 (insulinoma associated 2) gene family members are also direct target genes of Ngn3 gene, initially proving that INSM1 / INSM2 gene may play an important regulatory role in pancreatic endocrine development, further supplementing Perfect Ngn3 transcriptional regulatory network. Therefore, a deep understanding of the molecular regulation mechanism of Ngn3 gene and its transcriptional regulatory networks, especially INSM1 / INSM2 gene, on islet development and mature islet cell fate will be helpful to study the pathogenesis of endocrine and endocrine diseases of pancreas, especially diabetes, Treatment and prevention. For this reason, the research progress of Ngn3 gene and its transcriptional regulation network in recent years is reviewed.