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高胆固醇诱导家兔形成动脉粥样硬化已成为研究动脉粥样硬化形成机制的常用方法。在探求动脉粥样硬化形成的生化机理过程中,发现除了高LDL、高胆固醇血症外,动脉壁的C-AMP含量下降,铜兰旦白升高,5′-核苷酸酶下降,动脉壁的胆固醇含量在与正常组比较明显升高(P<0.05)。在以往的研究中,比较重视观察血管壁损伤、免疫复合物形成,血浆脂旦白等,但较少重视能量代谢的变化。依我室的实际情况,首先制备了红细胞膜,并进行了膜的生化分析。继以前报告又利用我室常规的单一显色剂方法测定了家兔正常与动脉粥样硬化形成时红
High cholesterol-induced rabbit atherosclerosis has become a commonly used method to study the mechanism of atherosclerosis. In exploring the biochemical mechanism of atherosclerosis, it was found that in addition to high LDL, hypercholesterolemia, the C-AMP content of the arterial wall decreased, copper lantern increased, 5’-nucleotidase decreased, arteries Wall cholesterol levels were significantly increased compared with the normal group (P <0.05). In previous studies, more attention was paid to the observation of vascular wall injury, immune complex formation, plasma lipoprotein, etc., but less attention was paid to changes in energy metabolism. According to the actual situation in our room, we first prepared the erythrocyte membrane and performed the biochemical analysis of the membrane. Following the previous report and the use of our room routine single reagent method was determined in rabbits normal and atherosclerosis when red