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[目的]为了解RS261菌株诱导辣椒抗疫病的生理生化机理。[方法]测定用RS261菌株处理后辣椒果内可溶性蛋白、丙二醛(MDA)含量及过氧化氢酶(POD)、超氧化物歧化酶(SOD)、苯丙氨酸解氨酶(PAL)活性的变化。[结果]RS261菌株对辣椒苗可溶性蛋白含量和防御性酶活性无不良影响。疫霉和菌液+疫霉2个处理的辣椒苗MDA含量及SOD、CAT和POD活性在处理后呈不断上升趋势,只是菌液+疫霉处理的上升量比疫霉处理的低,而可溶性蛋白含量和PAL活性变化呈现不同趋势,菌液+疫霉处理的辣椒苗内可溶性蛋白含量和PAL活性随时间推移不断上升,而仅接病菌处理却不断下降。[结论]RS261菌株可以在一定程度上消除病原菌对辣椒体内活性氧清除相关酶(SOD、CAT和POD)的酶活性,共同接种后可诱导寄主体内PAL活性的变化,从而提高寄主的抗性。
[Objective] The aim of the study was to understand the physiological and biochemical mechanism of RS261 induced resistance to pepper disease. [Method] The contents of soluble protein and malondialdehyde (MDA), catalase (POD), superoxide dismutase (SOD) and phenylalanine ammonia lyase (PAL) Changes in activity. [Result] RS261 strain had no adverse effect on soluble protein content and defensive enzyme activity in pepper seedlings. Phytophthora capsici and Phytophthora capsici 2 treatment of pepper seedlings MDA content and SOD, CAT and POD activity showed an upward trend after treatment, but the increase of fungus Phytophthora + Phytophthora treatment was lower, and soluble The changes of protein content and PAL activity showed different trends. The content of soluble protein and the activity of PAL in pepper seedlings treated by Phytophthora capsici and Phytophthora capsici increased with the passage of time, but only decreased with the treatment of pathogen only. [Conclusion] The strain RS261 can eliminate the enzyme activity of enzymes (SOD, CAT and POD) related to active oxygen scavenging of pepper by pathogen to some extent. After co-inoculation, RS261 strain can induce the change of PAL activity in host and enhance the host resistance.