论文部分内容阅读
为了证明肾综合征出血热纯化疫苗的主要成分为汉坦病毒蛋白 ,采用出血热纯化疫苗经浓缩后进行SDS PAGE和Western blotting分析。结果经SDS PAGE显示 ,肾综合征出血热纯化疫苗有三条蛋白带 ,分子量分别约为 70kD、5 5kD和 5 0kD ,与汉坦病毒三种结构蛋白 (糖蛋白G1、G2 和核蛋白NP)的分子量相符 ;经Western blotting显示 ,分子量 5 0kD的蛋白带反应阳性 ,分子量 70kD和 5 5kD的蛋白带无反应。认定出血热纯化疫苗的主要成分为汉坦病毒蛋白 ,主要由G1、G2 和NP三种结构蛋白构成。
In order to prove that the main component of the purified vaccine for hemorrhagic fever with renal syndrome is Hantavirus protein, the purified vaccine of hemorrhagic fever was concentrated and analyzed by SDS PAGE and Western blotting. Results SDS PAGE showed that there were three protein bands in the purified vaccine of hemorrhagic fever with renal syndrome, with molecular weights of about 70kD, 55kD and 50kD, respectively, which were significantly different from those of the three structural proteins of hantavirus (glycoproteins G1, G2 and NP) Western blotting showed that the protein bands with a molecular weight of 50 kD were positive and the bands with molecular weights of 70 kD and 55 kD were non-reactive. The main component of the haemorrhagic fever-purified vaccine is Hantavirus protein, which is mainly composed of three structural proteins G1, G2 and NP.