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目的:探讨组织蛋白酶B(CB)在子痫前期(PE)、子痫发病中的作用。方法:选择正常晚期妊娠妇女和子痫前期、子痫患者共70例,采用酶联免疫吸附实验检测血清中CB的水平,用免疫组织化学方法测定其中40例胎盘中CB的表达。结果:子痫前期、子痫患者血清中CB水平与正常晚孕组比较无明显变化(P>0.05),胎盘中CB主要表达于绒毛及蜕膜组织细胞及基质中,PE组胎盘表达CB较正常晚孕组明显增强(P<0.05),且与疾病的严重程度呈正相关;子痫患者胎盘中CB表达与重度子痫前期无明显差别(P>0.05)。结论:PE胎盘中CB表达增强可能是PE和子痫的发病机制之一。
Objective: To investigate the role of cathepsin B in preeclampsia (PE) and eclampsia. Methods: Seventy patients with normal late pregnant women and preeclampsia and eclampsia were selected. Serum levels of CB were measured by enzyme-linked immunosorbent assay (ELISA). The expression of CB in 40 placentas was detected by immunohistochemistry. Results: In preeclampsia, the level of CB in serum of eclampsia patients had no significant difference compared with that of normal pregnancy group (P> 0.05). CB in placenta was mainly expressed in villus and decidua cells and matrix. The normal pregnancy group was significantly enhanced (P <0.05), and with the severity of the disease was positively correlated; placental expression of CB in eclampsia and severe preeclampsia no significant difference (P> 0.05). Conclusion: The enhanced expression of CB in PE placenta may be one of the pathogenesis of PE and eclampsia.