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目的探讨早期妊娠和稽留流产患者外周血CD56brightCD16- NK细胞及蜕膜组织子宫自然杀伤(uNK)细胞中整合素α4(CD49d)和αm(CD11b)的表达。方法采集18例早期妊娠妇女外周血(其中14例另取蜕膜组织)和9例稽留流产妇女外周血,Ficoll密度梯度离心法分离单个核细胞悬液,流式细胞术检测样本CD56brightCD16- NK细胞中CD49d和CD11b表达。结果 CD49d和CD11b在早期妊娠蜕膜CD56brightCD16- NK细胞表达显著低于早期妊娠妇女外周血CD56brightCD16-NK细胞(P<0.01);稽留流产患者外周血CD56brightCD16- NK细胞的表达较早期妊娠妇女显著降低(P<0.05)。结论 CD49d和CD11b可能在妊娠早期uNK细胞的募集中起重要作用,同时也可能参与了正常妊娠的维持。
Objective To investigate the expression of integrin α4 (CD49d) and αm (CD11b) in peripheral blood CD56brightCD16-NK cells and decidual uterine natural killer (uNK) cells in patients with early pregnancy and missed abortion. Methods Peripheral blood samples of 18 pregnant women with early decidual tissue from 14 pregnant women and 9 aborted women were collected. The suspension of mononuclear cells was isolated by Ficoll density gradient centrifugation. CD56brightCD16-NK cells were detected by flow cytometry CD49d and CD11b expression. Results The expression of CD56b and CD16b in CD56brightCD16-NK cells of early pregnancy deciduals was significantly lower than that of CD56brightCD16-NK cells in early pregnant women (P <0.01). The CD56bright CD16-NK cells in peripheral blood of patients with missed abortion were significantly lower than those in early pregnant women P <0.05). Conclusion CD49d and CD11b may play an important role in the recruitment of uNK cells in early pregnancy, and may also be involved in the maintenance of normal pregnancy.