论文部分内容阅读
目的:制备顺铂载药纳米微球,并通过理化手段检测其各项特征,考察其体外对骨肉瘤细胞的杀伤效果。方法:合成PCL-PEG二嵌段共聚物,采用溶剂分散法制备顺铂载药纳米微球。测定该顺铂载药微球对人骨肉瘤U-20s细胞系的杀伤效果。结果:所制备的载药微球粒径<100 nm。载药量在5%左右,包封率则超过80%。体外稳定性实验证实了顺铂微球在室温下具有良好的稳定性。体外释放曲线显示了顺铂微球良好的缓释特性。MTT实验显示顺铂载药微球同样具有浓度依赖性的细胞杀伤效果,其各个时间点IC50值均稍大于顺铂裸药。结论:顺铂载药纳米微球具有一定的抗骨肉瘤作用,该种顺铂纳米给药系统作为一种新型的抗骨肉瘤纳米制剂值得进一步研究。
OBJECTIVE: To prepare cisplatin-loaded nanospheres by physical and chemical means and to examine its killing effect on osteosarcoma cells in vitro. Methods: PCL-PEG diblock copolymer was synthesized and the drug-loaded nanospheres were prepared by solvent dispersion method. The killing effect of the cisplatin-loaded microspheres on the human osteosarcoma U-20s cell line was determined. Results: The diameter of prepared drug-loaded microspheres was less than 100 nm. Drug loading at about 5%, encapsulation efficiency is more than 80%. In vitro stability experiments confirmed that cisplatin microspheres have good stability at room temperature. The in vitro release profile shows good sustained-release properties of cisplatin microspheres. MTT experiments showed that cisplatin-loaded microspheres also had a concentration-dependent cytotoxicity effect, with IC50 values slightly greater than that of cisplatin nucleases at each time point. CONCLUSION: Cisplatin-loaded nanospheres have certain anti-osteosarcoma effect. The cisplatin-based nanosized drug delivery system, as a new type of anti-osteosarcoma nanomodulator, deserves further study.