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目的观察吡咯烷二硫代氨基甲酸酯(PDTC)对匹罗卡品(pilocarpine)诱导的大鼠癫痫发作行为及脑内NF-κB活化的抑制作用。方法以PDTC(10 mg/kg)干预大鼠后,再给予致痫剂量的匹罗卡品(30 mg/kg)诱导癫痫发作,观察并记录大鼠的癫痫发作行为,并用免疫组织化学染色方法检测NF-κB在大鼠脑内海马、杏仁核、梨状皮层、内嗅皮层、大脑皮层等癫痫相关脑区中神经元的表达。结果经PDTC干预的大鼠能明显抑制或降低匹罗卡品的致痫作用,并且抑制了癫痫相关脑区NF-κB的表达。结论 PDTC具有抑制大鼠癫痫发作和脑内NF-κB表达的作用,为进一步探讨PDTC对癫痫大鼠的脑保护作用提供实验基础和理论依据。
Objective To observe the inhibitory effect of pyrrolidine dithiocarbamate (PDTC) on pilocarpine-induced seizure behavior and NF-κB activation in the brain of rats. Methods The rats were induced by PDTC (10 mg / kg), and epileptic seizures were induced by pilocarpine (30 mg / kg). The seizure behaviors of rats were observed and recorded. Immunohistochemical staining The expression of NF-κB in hippocampus, amygdala, piriform cortex, entorhinal cortex, cerebral cortex and other epilepsy-related brain regions were detected. Results PDTC intervention could obviously inhibit or reduce the epilepsy induced by pilocarpine and inhibit the expression of NF-κB in epilepsy-associated brain regions. Conclusions PDTC can inhibit the epileptic seizures and the expression of NF-κB in the brain and provide the experimental basis and theoretical basis for further exploring the protective effect of PDTC on epileptic rats.