In the reconstruction of past climate using stable carbon isotope composition (δ13C) in tree ring, the responses of the stable carbon composition (δ13C) of multiple tree species to environmental factors must be known detailedly. This study presented two δ13C series in annual tree rings for Chinese hemlock (Tsuga chinensis Pritz) and alpine pine (Pinus densata Mast), and investigated the relationships between climatic parameters and stable carbon discrimination (Δ13C) series, and evaluated the potential of climatic reconstruction using Δ13C in both species, in a temperate-moist region of Chuanxi Plateau, China. The raw δ13C series of the two species was inconsistent, which may be a result of different responses caused by trees inherent physiological differences. After removing the low-frequency effects of CO2 concentration, the high-frequency (year-to-year) inter-series correlation of Δ13C was strong, indicating that Δ13C of the two tree species were controlled by common environmental conditions. The Δ13C series of the species were most significantly correlated with temperature and moisture stress, but in different periods and intensity between the species. During the physiological year, the impacts of temperature and moisture stress on Δ13C occur earlier for Chinese hemlock (previous December to February for moisture stress and February to April for temperature, respectively) than for alpine pine (March to May for moisture stress and April to July for temperature, respectively). In addition, in temperatemoist regions, the control on Δ13C of single climatic parameter was not strongly dominant and the optimal multiple regressions functions just explained the 38.5% variance of the total. Therefore, there is limited potential for using δ13C alone to identify clear, reliable climatic signals from two species.