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以黄瓜无侧枝品系419(P1)和有侧枝品系SB-2(P2)为亲本构建的136株F2群体为试验材料,对黄瓜无侧枝基因nlb进行定位研究。田间调查和遗传分析结果表明,F2群体的表现型中有侧枝与无侧枝的分离比为3︰1。运用分离群体分组混合分析法(bulked segregant analysis,BSA),从F2无侧枝群体和有侧枝群体中各随机选取10株,分别混合其DNA,构建无侧枝和有侧枝基因池,通过分析SSR分子标记在基因池间的多态性,筛选得到nlb基因连锁标记9个。然后利用F2群体进行进一步验证,经MAPMAKER3.0软件分析,将nlb基因定位于黄瓜1号染色体,其中距离nlb基因较为紧密的标记是SSR19673,遗传距离为15.9cM。
A total of 136 F2 populations, including the cucumber-free colony 419 (P1) and the collateralized line SB-2 (P2), were used as experimental materials to locate the nlb without collateral in cucumber. The results of field investigation and genetic analysis showed that the segregation ratio of the collateral and non-collateral in F2 population was 3︰1. Using bulked segregant analysis (BSA), 10 strains were randomly selected from F2 non-collateral groups and collateral groups, and their DNAs were mixed to construct colony without colony and collateral gene. By analyzing SSR molecular markers In the gene pool polymorphism, screening nlb gene linked markers 9. Then the F2 population was used for further validation. The nlb gene was located on the chromosome 1 of cucumber via MAPMAKER3.0 software. The closest marker to the nlb gene was SSR19673 with a genetic distance of 15.9 cM.