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利用60 Co-γ射线辐射籼稻品种93-11,获得了两份卷叶突变体rl3(t)-1和rl3(t)-2。与野生型93-11相比,两个突变体叶片显著内卷,株高降低,穗长变短,并且结实率降低。细胞学分析表明,突变体叶片中泡状细胞数量的减少和形态的改变,可能是导致叶片卷曲的原因之一。遗传分析和等位性测验表明突变体受一对隐性核基因控制,且rl3(t)-1和rl3(t)-2等位。以卷叶突变体rl3(t)-1与武运粳8号杂交的F2分离群体作为定位群体,利用SSR标记和新发展的STS标记,将RL3(t)基因初步定位于第3染色体长臂上STS标记M3-45和SSR标记RM6676之间,遗传距离分别为5.5和4.4cM。进一步扩大定位群体,结合新发展的STS分子标记,最终将该卷叶基因定位在STS标记S3-39和S3-36之间,该区段物理距离为46kb。
Two coil mutant rl3 (t) -1 and rl3 (t) -2 were obtained by irradiating indica rice variety 93-11 with 60 Co-γ ray. Compared with the wild type 93-11, the leaves of two mutants were remarkably reeled in, the plant height was decreased, the spike length was shortened, and the seed setting rate was decreased. Cytological analysis showed that the number of vacuolar cells in mutant leaves decreased and morphological changes may be one of the reasons leading to leaf curling. Genetic analysis and allelic tests showed that the mutant was controlled by a pair of recessive nuclear genes with rl3 (t) -1 and rl3 (t) -2 alleles. The F2 segregating population with the cross between the leaf-opening mutant rl3 (t) -1 and Wuyunjing 8 was used as the locus. The SSR marker and the newly developed STS marker were used to locate the RL3 (t) gene on the long arm of chromosome 3 The genetic distance between STS marker M3-45 and SSR marker RM6676 was 5.5 and 4.4 cM, respectively. Further expand the positioning of the population, combined with the newly developed STS molecular markers, and finally the curled leaf gene located between the STS markers S3-39 and S3-36, the physical distance of 46kb.