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目的对卢旺达产金盏菊花的化学成分进行研究,为进一步开发利用该植物资源提供依据。方法采用大孔吸附树脂、正相硅胶、Sephadex LH-20、反相ODS等柱色谱以及制备型高效液相色谱法等手段进行分离纯化,并通过理化性质与核磁共振等波谱学数据相结合的方法鉴定化合物的结构。结果从金盏菊花体积分数为70%的乙醇溶液提取物中分离鉴定了6个单体成分,分别为异鼠李素-3-O-芸香糖苷(isorhamnetin-3-O-rutinoside,1)、异鼠李素-3-O-新橙皮糖苷(isorhamnetin-3-O-neohespeidoside,2)、香蒲新苷(typhaneoside,3)、槲皮素-3-O-β-D-新橙皮糖苷(quercetin-3-O-β-D-neohespeidoside,4)、芦丁(rutin,5)、槲皮素-3-O-(2″,6″-二-O-α-L-鼠李糖基)-β-D-葡萄糖苷(quercetin-3-O-(2″,6″-di-O-α-L-rhamnopyranosyl)-β-D-glucopyranoside,6)。结论首次对东非产金盏菊干燥花的化学成分进行研究,分离得到了6个黄酮类化合物。
Objective To study the chemical constituents of the flower of Calendula officinalis in Rwanda and provide the basis for further exploitation and utilization of the plant resources. Methods Macroporous resin, normal phase silica gel, Sephadex LH-20, reversed-phase ODS column chromatography and preparative high performance liquid chromatography were used to separate and purify the compounds. The physicochemical properties and NMR spectroscopy data were combined Method to identify the structure of the compound. Results Six monomer components were isolated and identified from 70% ethanol solution of calendula officinalis, namely isorhamnetin-3-O-rutinoside (1) Isorhamnetin-3-O-neohespeidoside (2), typhaneoside (3), quercetin-3-O-β-D-neohesperidin quercetin-3-O-β-D-neohespeidoside (4), rutin (5), quercetin-3-O- (2 “, 6” -di-O-α-L-rhamnopyranosyl) -β-D-glucopyranoside, 6). Conclusions The chemical constituents of dry flowers of Calendula officinalis in eastern Africa were studied for the first time, and six flavonoids were isolated.