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目的通过对心脏在体心功能和离体心肌储备功能测定,评价粒细胞集落刺激因子(G-CSF)动员内皮祖细胞(EPCs)对心肌梗死(MI)大鼠心功能改善的作用。方法雄性Wistar大鼠36只,随机分为对照组、MI组和G-CSF组(每组12只),MI组和G-CSF组采用冠脉左前降支结扎法制作MI模型后,分别腹腔注射生理盐水和G-CSF连续5d。给药后第7天,球后静脉采血,测定EPCs的数目和血浆血管内皮生长因子(VEGF)及C反应蛋白(CRP)的浓度。第4周时,进行在体心功能和离体心肌储备功能的测定,对MI大鼠的心功能进行全面评价。结果G-CSF可增加外周血中EPCs的数目,提高血浆中VEGF的浓度。同时在体心功能和离体心肌储备功能测定的结果显示,MI大鼠的心功能得到改善。结论G-CSF能够促进心梗大鼠EPCs的动员,增强VEGF的释放,改善MI大鼠的心功能。因此,G-CSF用于EPCs动员治疗AMI在临床上是有应用前景的。
OBJECTIVE: To evaluate the effects of G-CSF-mobilized endothelial progenitor cells (EPCs) on cardiac function in myocardial infarction (MI) rats by measuring heart function in vivo and in vitro myocardial reserve function. Methods Thirty-six male Wistar rats were randomly divided into control group, MI group and G-CSF group (12 in each group). MI group and G-CSF group were given MI model by left anterior descending coronary artery ligation, Normal saline and G-CSF were injected for 5 days. On the 7th day after the administration, blood was collected from the posterior retrobulbar vein to determine the number of EPCs and the concentrations of plasma vascular endothelial growth factor (VEGF) and C-reactive protein (CRP). At week 4, heart function was measured in vitro and myocardial reserve function was measured, and heart function of MI rats was evaluated comprehensively. Results G-CSF increased the number of EPCs in peripheral blood and increased the concentration of VEGF in plasma. At the same time in the body function and isolated myocardial reserve function test results showed that heart function MI rats improved. Conclusion G-CSF can promote the mobilization of EPCs, enhance the release of VEGF and improve the cardiac function in MI rats. Therefore, G-CSF for the EPCs Mobilization of AMI is clinically promising.