对叔戊醇中酶法合成L 抗坏血酸棕榈酸酯 (L AP)的反应动力学进行了研究 ,对影响其反应动力学的因素 (转速、温度、水分活度、酶质量分数和底物浓度 )进行了探讨 ,确定了最有效的酶促反应环境 .即转速为 2 0 0r/min ,温度为 55℃ ,水分活度为 0 ,加酶量为 2 0 % .并对底物进行了比较 ,得到了对酶亲和力最大的底物———棕榈酸甲酯 . The reaction kinetics of enzymatic synthesis of L-ascorbyl palmitate (L AP) in t-amyl alcohol was studied. The factors influencing the reaction kinetics (speed, temperature, water activity, enzyme mass fraction and substrate concentration) The most effective enzymatic reaction environment was determined, that is, the rotation speed was 200r / min, the temperature was 55 ℃, the activity of water was 0 and the amount of enzyme was 20% .The substrates were compared, Get the most enzyme affinity substrate --- methyl palmitate.