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利用葡萄无核基因的特异探针１８ｂｐ,通过ＰＣＲ扩增获得了与葡萄无核基因相连锁的ＳＣＡＲ标记约５９０ｂｐ,采用自动荧光ＤＮＡ测序仪对该片段的核苷酸组成进行双向测序,来自无核白的无核基因特异标记由５６９对核苷酸组成。该标记可以作为合成探针和设计特异引物进行ＰＣＲ扩增的基础,用于检测葡萄育种材料和无核品种的无核性。
Using 18 bp specific probe of grape seedless gene, the SCAR marker linked to grape seedless gene was about 590 bp by PCR. The nucleotide composition of the fragment was bi-directionally sequenced by using autofluorescence DNA sequencer, The nucleus-free, nuclear-free gene-specific marker consists of 569 pairs of nucleotides. This marker serves as a basis for PCR amplification of synthetic probes and design-specific primers for the detection of seedlessness of grape breeding material and seedless varieties.