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目的 :研究充血性心力衰竭 (心衰 )患者血浆内皮素 (ET)水平和ET受体基因在其外周血单个核细胞表达情况。方法 :在 36例心衰 (心衰组 )患者和 12例健康人 (对照组 )中分别采用放免法测定血浆ET水平、逆转录聚合酶链反应检测ET受体基因在其外周血单个核细胞的表达。结果 :心衰组血浆ET水平〔(4 .90± 1.96 )pmol L〕明显高于对照组〔(1.76± 0 .82 )pmol L〕(P <0 .0 1)。ET受体基因表达阳性率在两组差异无显著性意义(P >0 .0 5 ) ,半定量后 ,心衰组ETA受体 (0 .82± 0 .2 6 )表达水平明显高于对照组 (0 .5 9± 0 .17) (P <0 .0 1)。结论 :ET及其A受体与心衰的发生、发展有密切关系
Objective: To investigate the level of plasma endothelin (ET) and the expression of ET receptor gene in peripheral blood mononuclear cells in patients with congestive heart failure (CHF). Methods: Plasma ET levels were determined by radioimmunoassay in 36 patients with heart failure (HF) and 12 healthy individuals (control group). The expression of ET receptor gene was detected by RT-PCR in peripheral blood mononuclear cells expression. Results: Plasma ET level in patients with heart failure 〔(4.90 ± 1.96) pmol L〕 was significantly higher than that in the control group 〔(1.76 ± 0.82) pmol L〕 (P0.01). The positive rate of ET receptor gene expression was not significantly different between the two groups (P> 0.05). After semi-quantitative analysis, the expression of ETA receptor (0.82 ± 0.26) in heart failure group was significantly higher than that of control Group (0.59 ± 0.17) (P <0.01). Conclusion: ET and its A receptor are closely related to the occurrence and development of heart failure