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目的探讨miR-143在结直肠癌中的表达情况及其与临床病理指标间关系。方法采用RT-PCR法检测miR-143在17例结肠癌患者的肿瘤标本(结肠癌组)及癌旁黏膜组织(结肠黏膜组)和81例直肠癌患者的肿瘤标本(直肠癌组)及癌旁黏膜组织(直肠黏膜组)标本中的表达情况,并分析其与结直肠癌患者临床病理指标间关系。结果结肠癌组miR-143表达水平(Ct值为15.96±1.76)明显低于结肠黏膜组(Ct值为14.34±3.44),差异有统计学意义(P=0.023),直肠癌组miR-143表达水平(Ct值为14.66±3.18)与直肠黏膜组(Ct值为14.29±4.82)比较,差异无统计学意义(P=0.351);直肠癌组miR-145表达下调在性别、年龄、TNM分期、脉管浸润上差异无统计学意义(P>0.05)。结论 miR-143仅在结肠癌中表达降低,在直肠癌中表达无变化,miR-143仅参与了结肠癌的发生,结肠癌和直肠癌可能有不同的癌变分子基础。
Objective To investigate the expression of miR-143 in colorectal cancer and its relationship with clinicopathological parameters. Methods RT-PCR was used to detect the expression of miR-143 in tumor specimens (colon cancer group) and adjacent non-cancerous tissue (colon mucosa group) and 81 rectal cancer patients in 17 colon cancer patients (rectal cancer group) and cancer Adjacent mucosa (rectal mucosa) specimens, and analyze the relationship between them and the clinicopathological parameters of patients with colorectal cancer. Results The expression of miR-143 in colon cancer group was significantly lower than that in colon mucosa (Ct = 15.96 ± 1.76) (Ct = 14.34 ± 3.44, P = 0.023) The level of miR-145 in rectal cancer was significantly lower than that in rectal mucosa (14.66 ± 3.18) and rectal mucosa (14.29 ± 4.82, P = 0.351) Vascular infiltration difference was not statistically significant (P> 0.05). Conclusion The expression of miR-143 is only decreased in colon cancer, but there is no change in rectal cancer. MiR-143 is only involved in the development of colon cancer. Colon cancer and rectal cancer may have different molecular basis of carcinogenesis.