论文部分内容阅读
目的对从广州白云国际机场口岸入境的1例自委内瑞拉归国发热人员进行寨卡病毒实验室检测。方法同时使用自主研制的和中国疾病预防控制中心发放的实时荧光RT-PCR检测试剂,对发热人员的血清、全血、尿液、唾液和咽拭子等多种样本进行寨卡病毒核酸检测;对唾液样本进行寨卡病毒RT-PCR扩增和序列分析。结果入境第一天上午采集的血清、全血和咽拭子样本的实时荧光RT-PCR结果显示寨卡病毒核酸阳性,当天下午采集的血清样本检测结果显示核酸弱阳性,第二天采集的血清样本为寨卡病毒核酸阴性,但尿液和唾液样本仍为阳性,且唾液中病毒载量较高。RT-PCR结果显示,唾液样本扩增出了预期片段,测序结果表明该片段与巴西寨卡病毒毒株相应序列的同源性为99.8%。中国疾病预防控制中心复核检测确认为寨卡病毒核酸阳性。结论根据流行病学史、临床表现和病例样本核酸检测情况,确诊该入境人员为口岸截获首例、广东省首例、我国第二例输入性寨卡病毒感染病例。
Objective To test Zika virus in a case of fever returning from Venezuela which entered the port of Guangzhou Baiyun International Airport. Methods Real-time fluorescent RT-PCR reagents developed independently by China CDC were used to detect Zika virus nucleic acid in various samples such as serum, whole blood, urine, saliva and throat swabs of febrile persons. Sika samples were subjected to Zika virus RT-PCR amplification and sequence analysis. Results Real-time fluorescence RT-PCR results of serum, whole blood and throat swab samples collected on the morning of the first day of entry showed that Zika virus nucleic acid was positive. The serum samples collected in the afternoon showed that the nucleic acids were weakly positive. The serum collected the next day Samples were negative for Zika virus nucleic acid, but urine and saliva samples were still positive, and salivary viral load was high. The results of RT-PCR showed that the expected fragment was amplified from the salivary samples, and the sequence was 99.8% homologous to the corresponding sequence of Zika virus in Brazil. The Chinese Center for Disease Control and Prevention check the test confirmed as Zika virus nucleic acid positive. Conclusions According to epidemiological history, clinical manifestations and case-detected nucleic acid detection, the first case of port entry was confirmed. The first case of Guangdong Province and the second case of imported Zika virus infection in China.