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目的探讨哺乳期母鼠接触溴氰菊酯(DM)对发育期仔鼠脑一氧化氮合酶(NOS)活力及神经行为发育的影响。方法妊娠大鼠随机分为对照组和2个染毒组,每组6只。染毒剂量分别为3．35和6．70 mg/kg,每只母鼠自产后1～19 d,隔日1次,经口染毒DM；对照组予等量玉米油。观察DM对出生后5、10和21 d仔鼠脑NOS活力及30d仔鼠学习、记忆能力的影响。结果2个染毒组仔鼠哺乳存活率(81．80%、78．60%)均明显降低于对照组(96．70%),差异有统计学意义(P＜0．01)；6．70 mg/kg染毒组10、21 d仔鼠体重[(16．62±2．28)、(31．34±6．94)g]明显低于对照组[(18．81±2．01)、(36．21±7．01)g],差异有统计学意义(P＜0．05)；6．70mg/kg染毒组仔鼠延迟时间为(3．05±1．20)s,被动逃避反应阳性率为21．5%；3．35 mg/kg染毒组被动逃避反应阳性率为22．5%,与对照组比较,差异均有统计学意义(P＜0．05)。旷场实验中,2个染毒组仔鼠行进格子数均明显降低,与对照组的差异均有统计学意义(P＜0．05)；产后5至21 d仔鼠脑NOS活力呈发育性增高趋势；6．70mg/kg染毒组出生后5 d仔鼠脑NOS活力[(0．60±0．07)U·mg pro~(-1)·h~(-1)]明显低于对照组,差异有统计学意义(P＜0．05)结论哺乳期母鼠接触DM可导致仔鼠神经行为发育迟缓,并伴有NOS活力下降。
Objective To investigate the effects of exposure to deltamethrin (DM) on the activity of nitric oxide synthase (NOS) and the neurobehavioral development of the brain in neonatal rats during lactation. Methods Pregnant rats were randomly divided into control group and two exposure groups, 6 rats in each group. The exposure doses were 3.35 and 6.70 mg / kg, and each mother was given 1 to 19 days after birth and once every other day. The rats in the control group were given the same amount of corn oil. To observe the effects of DM on brain NOS activity and learning and memory abilities of offspring rats on the 5th, 10th and 21st day after birth. Results The surviving rate (81.80%, 78.60%) in the two exposure groups were significantly lower than that in the control group (96.70%) (P <0.01) .6. The body weight of the offspring in the 70 mg / kg and 10,21 d [(16.62 ± 2.28) and (31.34 ± 6.94) g] groups was significantly lower than that in the control group [(18.81 ± 2.01 ), (36.21 ± 7.01) g], the difference was statistically significant (P <0.05); the delay time of 6.70mg / kg exposure group was (3.05 ± 1.20) s , And the positive rate of passive evasion was 21.5%. The positive rate of passive evasion of 3.35 mg / kg was 22.5%, which was significantly different from the control group (P <0.05) . In the open-field test, the number of advancing plaques in two exposed groups was significantly lower than that in the control group (P <0.05), and the NOS activity in the offspring from the 5th to 21st postpartum was developmental (0.60 ± 0.07) U · mg pro ~ (-1) · h ~ (-1)] in the 6.70mg / kg group were significantly lower than The difference was statistically significant (P <0.05) .Conclusion Exposure to DM in nursing mothers may lead to neurobehavioral retardation in the offspring with decreased NOS activity.