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目的应用分离培养法与巢式聚合酶链反应法(PCR)检测深圳环境水中军团菌最新污染状况。方法于2012年9-11月采集商场、宾馆和综合性医院三类场所的中央空调冷却水、淋浴水、自来水、景观水,分别用传统分离培养法与巢式聚合酶链反应法(PCR)检测嗜肺军团菌。结果本次共检测环境水样171份,其中传统分离培养法检出64份嗜肺军团菌,巢式聚合酶链反应法(PCR)检出130份嗜肺军团菌,水样的阳性率分别为37.4%和76%。结论深圳多种水源存在军团菌污染;巢式聚合酶链反应法(PCR)检出率高于传统分离培养法。
Objective To detect the latest contamination status of Legionella in Shenzhen’s environmental water by using isolation culture method and nested polymerase chain reaction (PCR). Methods The air-conditioned central air-conditioning cooling water, shower water, tap water and landscape water were collected in the shopping centers, hotels and general hospitals from September to November in 2012. The traditional methods of separation culture and nested polymerase chain reaction (PCR) Legionella pneumophila detection. Results A total of 171 environmental water samples were detected, of which Legionella pneumophila 64 were detected by traditional isolation culture method and 130 Legionella pneumophila were detected by nested polymerase chain reaction (PCR). The positive rates of water samples were 37.4% and 76%. Conclusion Legionella contamination was found in many water sources in Shenzhen. The detection rate of nested polymerase chain reaction (PCR) was higher than that of traditional isolation culture method.