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研究胆固醇对胆道口括约肌(SO)细胞动力改变的结构基础,进一步解释胆固醇结石形成的原因.方法:分离幼兔SO采用复合酶消化法行原代培养α-平滑肌肌动蛋白(α-SMActin)染色鉴定,人工制作胆固醇脂质体(CL),按不同浓度作用细胞后行显微镜、原位包埋电镜观察及电泳测定.结果:电镜示CL组部分细胞器肿胀、扩张,微丝有明显分开、减少的趋势,电泳示随胆固醇浓度增高细胞蛋白(包括Actin)含量减少.结论:胆固醇首先作用于SO细胞膜,改变其流动性及通透性,继而可以直接作用于细胞器及微丝结构,通过改变细胞骨架的构型及含量而引起SO动力改变.
To study the structural basis of the dynamic changes of cholesterol in the biliary sphincter (SO) cells and to further elucidate the causes of cholesterol gallstone formation. Methods: SO in rabbits was isolated and identified by α-SMActin staining using enzyme digestion method. Cholesterol liposomes (CL) were artificially produced. The cells were exposed to different concentrations of streptomycin, Embedding electron microscopy and electrophoresis. Results: Electron microscopy showed some of the organelles in CL group swollen and expanded, and the microfilaments were obviously separated and decreased. Electrophoresis showed that the content of cellular proteins (including Actin) decreased with the increase of cholesterol concentration. CONCLUSION: Cholesterol first acts on the membrane of SO cells and changes its fluidity and permeability. It can directly act on organelle and microfilament structure and change SO dynamic by changing the configuration and content of cytoskeleton.