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目的:探讨抗原抗体反应检验若干人肿瘤细胞分泌β-hCG情况,和抗hCGCP22抗血清体外抑制肿瘤的作用。方法:采用细胞免疫荧光染色测定5个肿瘤细胞株是否表达β-hCG,用CCK-8试剂盒检测CP22抗血清(AS CP22)和β-hCG羧基端37肽(CTP)抗血清(AS CTP)体外抑制肿瘤细胞生长的光密度。结果:以AS CP22为探针和AS CTP为阳性对照,体外培养的人胰腺癌BXPC-3、人卵巢癌HO-8910和人结肠癌Lovo细胞膜上都能观察到绿色荧光,提示肿瘤细胞均表达β-hCG亚单位,而人前列腺癌PC-3和Du-145细胞则未检测到绿色荧光;与正常兔血清(NS)比较,AS CP22和AS CTP均可显著的体外抑制BXPC-3和HO-8910细胞生长,P<0.001。AS CP22抑制BXPC-3和HO-8910肿瘤细胞的作用都强于AS CTP,P<0.05。结论:人胰腺癌BXPC-3、人卵巢癌HO-8910和人结肠癌Lovo细胞在体外培养的情况下均表达β-hCG亚单位,且它们的体外生长都能被兔抗hCG嵌合肽(CP22)抗血清体外抑制。
OBJECTIVE: To investigate the effect of antigen-antibody reaction on the secretion of β-hCG by several human tumor cells and the anti-tumor effect of anti-hCGCP22 antiserum in vitro. Methods: The expression of β-hCG in 5 tumor cell lines was determined by immunofluorescence staining. The antiserum against AS-C22 and anti-β-hCG CTP were detected by CCK-8 kit. In vitro inhibition of tumor cell growth optical density. Results: Using AS CP22 as probe and AS CTP as positive control, green fluorescence was observed on human pancreatic cancer BXPC-3, human ovarian cancer HO-8910 and human colon cancer Lovo cells in vitro, suggesting that all the tumor cells were expressed β-hCG subunit. However, green fluorescence was not detected in human prostate cancer PC-3 and Du-145 cells. Compared with normal rabbit serum (NS), both AS CP22 and AS CTP significantly inhibited BXPC-3 and HO -8910 cell growth, P <0.001. The effect of AS CP22 on inhibiting BXPC-3 and HO-8910 tumor cells was stronger than AS CTP, P <0.05. CONCLUSION: The β-hCG subunits are expressed in human pancreatic cancer BXPC-3, human ovarian cancer HO-8910 and human colon carcinoma Lovo cells cultured in vitro, and their in vitro growth can be inhibited by rabbit anti-hCG chimeric peptide CP22) antiserum in vitro.